Journal: The Journal of International Medical Research

Article Title: Upregulation of matrix metalloproteinase 9 (MMP9)/tissue inhibitor of metalloproteinase 1 (TIMP1) and MMP2/TIMP2 ratios may be involved in lipopolysaccharide-induced acute lung injury

doi: 10.1177/0300060520919592

Figure Lengend Snippet: Primer sequences for real-time quantitative polymerase chain reaction analyses.

Article Snippet: Mouse anti-rat MMP9 (cat. no. NBP2-13173SS) and TIMP2 antibodies (cat. no. NBP1-42375) were purchased from Novus Biologicals Inc. (Littleton, CO, USA).

Techniques: Real-time Polymerase Chain Reaction

Journal: The Journal of International Medical Research

Article Title: Upregulation of matrix metalloproteinase 9 (MMP9)/tissue inhibitor of metalloproteinase 1 (TIMP1) and MMP2/TIMP2 ratios may be involved in lipopolysaccharide-induced acute lung injury

doi: 10.1177/0300060520919592

Figure Lengend Snippet: Lung MMP and TIMP mRNA and protein expression. Changes in MMP2 (a), TIMP2 (b), MMP2/TIMP2 (c), MMP9 (d), TIMP1 (e), and MMP9/TIMP1 (f) in all groups treated with or without LPS. (g) Protein expression in each group as detected by western blotting. LPS 2h, 6h, 12h, and 24h indicate 2, 6, 12, and 24 hours after LPS injection in the acute lung injury group, respectively. Data are shown as mean ± standard deviation (n = 6 per group). * P < 0.05 vs. control, # P < 0.05 vs. LPS 2h. Control, normal control group; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase; LPS, lipopolysaccharide

Article Snippet: Mouse anti-rat MMP9 (cat. no. NBP2-13173SS) and TIMP2 antibodies (cat. no. NBP1-42375) were purchased from Novus Biologicals Inc. (Littleton, CO, USA).

Techniques: Expressing, Western Blot, Injection, Standard Deviation, Control

Journal: Cureus

Article Title: Abnormal Trophoblast Invasion in Early-Onset Preeclampsia: The Involvement of Cystathionine β-Synthase, Specificity Protein 1 and microRNA-22

doi: 10.7759/cureus.104353

Figure Lengend Snippet: mRNA expression of matrix metalloproteinases (MMPs) 2, 9 (a) and tissue inhibitors of metalloproteinase (TIMPs) 1, 2 (b) in the placental tissue samples from pregnancies complicated by early-onset preeclampsia (EOPE). Bar diagrams represent the relative mRNA expression of MMPs 2, 9 (a) and TIMPs 1,2 (b) in placentae from EOPE and normotensive, non-proteinuric controls. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a positive control. Data presented as mean ± SEM. Wilcoxon matched-pairs signed rank (MMPs 2, 9, TIMP-1) and paired t (TIMP-2) tests were applied, *p≤0.05 was considered statistically significant, *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001, ns: not significant.

Article Snippet: Primary antibodies to MMP-2 (Abcam) at a dilution of 1:500, MMP-9 (Abcam) at a dilution of 1:500, TIMP-1 (Thermo) at a dilution of 1:100, TIMP-2 (Novus Biologicals) at a dilution of 1:100, CBS (Abcam) at a dilution of 1:400 and Sp1 (Merck) at a dilution of 1:250 were used.

Techniques: Expressing, Positive Control

Journal: Cureus

Article Title: Abnormal Trophoblast Invasion in Early-Onset Preeclampsia: The Involvement of Cystathionine β-Synthase, Specificity Protein 1 and microRNA-22

doi: 10.7759/cureus.104353

Figure Lengend Snippet: Protein expression of matrix metalloproteinases (MMPs) 2, 9, and their feedback inhibitor tissue inhibitors of metalloproteinase (TIMPs) 1, 2 as analysed by immunohistochemistry (IHC). Representative IHC images of placentae from early-onset preeclampsia (EOPE) (a, e, i, m) and normotensive, non-proteinuric controls (b, f, j, n) showing MMP-2, MMP-9, TIMP-1 and TIMP-2 localization in syncytiotrophoblasts, stromal component, and blood vessels. Positive controls for MMP-2 (human placenta (c)), MMP-9 (human spleen (g)), TIMP-1 (rat brain (k)) and TIMP-2 (human pancreas (o)). Negative controls for MMP-2 (d), MMP-9 (h), TIMP-1 (l) and TIMP-2 (p), Scale Bar: 50 µm.

Article Snippet: Primary antibodies to MMP-2 (Abcam) at a dilution of 1:500, MMP-9 (Abcam) at a dilution of 1:500, TIMP-1 (Thermo) at a dilution of 1:100, TIMP-2 (Novus Biologicals) at a dilution of 1:100, CBS (Abcam) at a dilution of 1:400 and Sp1 (Merck) at a dilution of 1:250 were used.

Techniques: Expressing, Immunohistochemistry

Journal: Cureus

Article Title: Abnormal Trophoblast Invasion in Early-Onset Preeclampsia: The Involvement of Cystathionine β-Synthase, Specificity Protein 1 and microRNA-22

doi: 10.7759/cureus.104353

Figure Lengend Snippet: Protein expression of matrix metalloproteinases (MMPs) 2, 9 and tissue inhibitors of metalloproteinase (TIMPs) 1, 2 as analysed by immunofluorescence (IF) in EOPE and normotensive controls. Representative IF images of placentae from EOPE and normotensive, non-proteinuric controls showing MMP-2 (a, c (FITC stained), b, d (merged)], MMP-9 (e, g (FITC stained), f, h (merged)), TIMP-1 (i, k (TRITC stained), j, l (merged)) and TIMP-2 (m, o (FITC stained), n, p (merged)) localization in syncytiotrophoblasts, stromal component and blood vessels. Nuclei were stained by 4′,6-diamidino-2-phenylindole (DAPI); Scale Bar: 50 µm, FITC: Fluorescein isothiocyanate, TRITC: Tetramethylrhodamine isothiocyanate.

Article Snippet: Primary antibodies to MMP-2 (Abcam) at a dilution of 1:500, MMP-9 (Abcam) at a dilution of 1:500, TIMP-1 (Thermo) at a dilution of 1:100, TIMP-2 (Novus Biologicals) at a dilution of 1:100, CBS (Abcam) at a dilution of 1:400 and Sp1 (Merck) at a dilution of 1:250 were used.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Cureus

Article Title: Abnormal Trophoblast Invasion in Early-Onset Preeclampsia: The Involvement of Cystathionine β-Synthase, Specificity Protein 1 and microRNA-22

doi: 10.7759/cureus.104353

Figure Lengend Snippet: Immunoblot of matrix metalloproteinases (MMPs) 2, 9 (a, b) and tissue inhibitors of metalloproteinase (TIMPs) 1, 2 (c, d) in the placentae from early-onset preeclampsia (EOPE) as compared to normotensive, non-proteinuric control placentae. Representative images of immunoblot showing the protein expression of MMP-2 (a), MMP-9 (b), TIMP-1 (c) and TIMP-2 (d) in placental tissues of EOPE and normotensive, non-proteinuric controls. Bar diagrams represent the normalized values of MMPs 2, 9 (e) and TIMPs 1, 2 (f) with respect to β-actin (loading control). Data presented as mean ± SEM. Statistical analysis was done using the Wilcoxon matched-pairs signed rank (MMPs 2, 9, TIMP-1) and paired t (TIMP-2) tests; *p≤0.05 was considered statistically significant. *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001, ns: not significant

Article Snippet: Primary antibodies to MMP-2 (Abcam) at a dilution of 1:500, MMP-9 (Abcam) at a dilution of 1:500, TIMP-1 (Thermo) at a dilution of 1:100, TIMP-2 (Novus Biologicals) at a dilution of 1:100, CBS (Abcam) at a dilution of 1:400 and Sp1 (Merck) at a dilution of 1:250 were used.

Techniques: Western Blot, Control, Expressing